Chinese Professional Cdmo Drug Development - Cytochrome P450 monooxygenase (CYP) – SyncoZymes

Catalytic reaction type:

★ Broad substrate spectrum.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution (The optimum reaction pH of enzyme), coenzyme (NAD(H) or NADP(H)), coenzyme regeneration system (e.g. glucose and glucose dehydrogenase) and the ES-CYP. coenzyme and coenzyme regeneration system could be replaced by hydrogen peroxide in partial reaction system.
➢ All kinds of ES-CYPs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-CYP’s activity. However, the inhibition can be relieved by batch addition of substrate.

Example 1 ⁽¹⁾:

Example 2 ⁽²⁾:

Example 3 ⁽³⁾:

Example 4⁽⁴⁾:

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.

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