Factory source The Best Nmn Supplement - Hydroaminase (HAM) – SyncoZymes

The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
10% (v/v): Ammonia
Adjust pH to 9.0 before adding enzyme, and enzyme should be added finally. Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.

Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.