Massive Selection for Resilience Cdmo - Nitrile hydratase (NHT) – SyncoZymes

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Massive Selection for Resilience Cdmo - Nitrile hydratase (NHT) – SyncoZymes Detail:

Nitrile hydratase NHT

Enzymes	Product Code	Specification
Enzyme Powder	ES-NHT-101~ ES-NHT-124	a set of 24 Nitrile Hydratases, 50 mg each 24 items * 50mg / item, or other quantity
Screening Kit (SynKit)	ES-NHT-2400	a set of 24 Nnitrile Hydratases, 1 mg each 24 items * 1mg / item

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution (optimum reaction pH) and ES-NHT.
➢ All the ES-NHTs can be tested respectively in the reaction system above or with the NHT Screening Kit (SynKit NHT).
➢ All kinds of ES-NHTs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-NHT’s activity. However, the inhibition can be relieved by batch addition of substrate, coupling with the amidases or product extraction.

Example 1(coupling with the amidase)⁽¹⁾:

Nitrile hydratase NHT3

Example 2⁽²⁾:

Nitrile hydratase NHT4

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.

1 Vojtech V, Ludmila M, Alicja B V, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal. Tetrahedron lett, 2010, 51: 1639-1641.

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