Newly Arrival Nicotinamide Mononucleotide And Resveratrol - Cytochrome P450 monooxygenase (CYP) – SyncoZymes

Catalytic reaction type:

★ Broad substrate spectrum.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution (The optimum reaction pH of enzyme), coenzyme (NAD(H) or NADP(H)), coenzyme regeneration system (e.g. glucose and glucose dehydrogenase) and the ES-CYP. coenzyme and coenzyme regeneration system could be replaced by hydrogen peroxide in partial reaction system.
➢ All kinds of ES-CYPs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-CYP’s activity. However, the inhibition can be relieved by batch addition of substrate.

Example 1 ⁽¹⁾:

Example 2 ⁽²⁾:

Example 3 ⁽³⁾:

Example 4⁽⁴⁾:

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.

1. Zaretzki J, Matlock M, and Swamidass S J. J. Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J,  Perez F A., e tal. J. Am. Chem. Soc., 2006, 128 (26),  8374–8375.
3. Cryle M J.,  Matovic N J., and  De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, W. K. e tal. Acta Crystallogr. 2009, 65, 372.