Popular Design for Pure Nicotinamide Mononucleotide - Leucine Dehydrogenase (LeuDH) – SyncoZymes

The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.

Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.