Quality Inspection for Nad To Nadph - Lactate Dehydrogenase (LDH) – SyncoZymes

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Quality Inspection for Nad To Nadph - Lactate Dehydrogenase (LDH) – SyncoZymes Detail:

Lactate Dehydrogenase LDH Instructions

Enzymes	Product Code	Specification
Enzyme Powder	ES-LDH-101	a set of 1 Monoamine Oxidase, 50 mg each 1 items * 50mg / item, or other quantity

The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.

Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.

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