Renewable Design for Apis Cdmo - Esterase&Lipase (PLE&CALB) – SyncoZymes

There are 26 kinds of PLE enzyme products (Number as ES-PLE-101～ES-PLE-126) developed by SyncoZymes. ES-PLE can be used for the hydrolysis of aliphatic and ester compounds, or regioselective and stereoselective resolution to synthesize chiral acids and their derivatives.

Catalytic reaction type:

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution and the ES-PLE. The esterification of some ES-PLEs is carried out in the organic phase.
➢ All kinds of ES-PLEs corresponding to various optimum reaction conditions should be studied individually.

Example 1(Biosynthesis of Pregabalin Intermediate) ⁽¹⁾:

Example 2 ⁽²⁾:

Example 3 ⁽³⁾:

Example 4⁽⁴⁾:

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH.

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4. Gais, H. J., Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917