Special Design for Beta Nadp - Lactate Dehydrogenase (LDH) – SyncoZymes
Special Design for Beta Nadp - Lactate Dehydrogenase (LDH) – SyncoZymes Detail:
| Enzymes | Product Code | Specification |
| Enzyme Powder | ES-LDH-101 | a set of 1 Monoamine Oxidase, 50 mg each 1 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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By Gill from London - 2017.09.29 11:19
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