Well-designed Nadph 4na - Cytochrome P450 monooxygenase (CYP) – SyncoZymes

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Well-designed Nadph 4na - Cytochrome P450 monooxygenase (CYP) – SyncoZymes Detail:

Catalytic reaction type:

cyp
cyp1
cyp2
cyp3

Enzymes	Screening Kit (SynKit)	Specification
Enzyme Powder	ES-CYP-101~ ES-CYP-108	a set of 8 Cytochrome P450 Monooxygenases, 50 mg each 8 items * 50mg / item, or other quantity
Screening Kit (SynKit)	ES-CYP-800	a set of 8 Cytochrome P450 Monooxygenases,1mg each 8 items * 1mg / item

★ Broad substrate spectrum.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution (The optimum reaction pH of enzyme), coenzyme (NAD(H) or NADP(H)), coenzyme regeneration system (e.g. glucose and glucose dehydrogenase) and the ES-CYP. coenzyme and coenzyme regeneration system could be replaced by hydrogen peroxide in partial reaction system.
➢ All kinds of ES-CYPs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-CYP’s activity. However, the inhibition can be relieved by batch addition of substrate.

Example 1 ⁽¹⁾:

cpyExample1

Example 2 ⁽²⁾:

cpyExample2

Example 3⁽³⁾:

cpyExample3

Example 4⁽⁴⁾:

cpyExample4

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.

1. Zaretzki J, Matlock M, and Swamidass S J. J. Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Chem. Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., and De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, W. K. e tal. Acta Crystallogr. 2009, 65, 372.

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David Sinclair Nicotinamide Riboside