Leucine Dehydrogenase (LeuDH)
Short Description:
About Leucine dehydrogenase
There is 1 kind of LeuDH enzyme product (Number as ES-LeuDH-101) developed by SyncoZymes. LeuDH can catalyze the reductive ammoniation of 4-methylbutanoic acid (salt) to L-leucine or the oxidation of leucine to 4-methylbutanoic acid. ES-LeuDH can also catalyze the reductive amination of α-keto acids with similar structure to prepare corresponding amino acid derivatives. In the process of catalysis, NADH is necessary as hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
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| Enzymes | Product Code | Specification |
| Enzyme Powder | ES-LeuDH-101 | a set of 1 Monoamine Oxidase, 50 mg each 1 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
