Imine reductase (IRED)
Short Description:
About Imine reductase
ES-IRED: A class of enzymes that catalyze the reduction of C=N bond to C-N bond. It belongs to redox enzyme, with coenzyme NADPH for hydrogen transportation. According to the chiral preference of the products, they can be divided into R-IRED and S-IRED. Syncozymes developed 15 imine reductases (numbIRED ES-IRED-101-ES-IRED-115), which can be widely used in regioselective and stereoselective reduction of imines to prepare chiral amines.
Catalytic reaction type:
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| Enzymes | Product Code | Specification |
| Enzyme Powder | ES-IRED-101~ ES-IRED-114 | a set of 14 Imine Reductase, 50 mg each |
| 96-Well Enzyme Screening Kit | ES-IRED-1400 | a set of 14 Imine Reductase, 1 mg each |
★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.
➢ Normally, the reaction system should include substrate, buffer solution, enzyme, coenzyme, and coenzyme regeneration system.
➢ All kinds of ES-IREDs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-IRED’s activity. However, the inhibition can be relieved by batch addition of substrate.
Example 1(Biosynthesis of chiral 2-methyl pyrrolidine)(1):
Example 2(Biosynthesis of secondary amine)(2):
Example 3 (Reduction of Cyclic Imines)(3):
Keep 2 years below -20℃.
Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.
1. Scheller PN, Fademrecht S, Hofelzer S, et al. ChemBioChem, 2014, 15, 2201-2204.
2. Wetzl D, Gand M, Ross A, et al. ChemCatChem, 2016, 8, 2023-2026.
3. Li H, Luan ZJ, Zheng GW, et al. Adv. Synth. Catal. 2015, 357, 1692-1696.
