Nitrile hydratase (NHT)

Short Description:

About ES-NHTs

ES-NHTs: a class of metalloenzyme, which selectively hydrate nitriles into the corresponding primary amide. They are widely used not only for the synthesis of amino acids, amides, carboxylic acids and their derivatives, but also for the preparation of chiral drugs. There are 24 kinds of nitrile hydratases developed by SyncoZymes (Number as ES-NHT-101~ES-NHT-124).
Catalytic reaction type:

Nitrile hydratase NHT2

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Product Detail

Product Tags

Product Information:

Nitrile hydratase NHT
Enzymes Product Code Specification
Enzyme Powder ES-NHT-101~ ES-NHT-124 a set of 24 Nitrile Hydratases, 50 mg each 24 items * 50mg / item, or other quantity
Screening Kit (SynKit) ES-NHT-2400 a set of 24 Nnitrile Hydratases, 1 mg each 24 items * 1mg / item


★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

Instructions for use:

➢ Normally, the reaction system should include substrate, buffer solution (optimum reaction pH) and ES-NHT.
➢ All the ES-NHTs can be tested respectively in the reaction system above or with the NHT Screening Kit (SynKit NHT).
➢ All kinds of ES-NHTs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-NHT’s activity. However, the inhibition can be relieved by batch addition of substrate, coupling with the amidases or product extraction.

Application Examples:

Example 1(coupling with the amidase) (1):

Nitrile hydratase NHT3

Example 2 (2):

Nitrile hydratase NHT4


Keep 2 years below -20℃.


Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.


1 Vojtech V, Ludmila M, Alicja B V, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal. Tetrahedron lett, 2010, 51: 1639-1641.

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