Nitrilase (NIT)

Short Description:

About Nitrilase

ES-NITs: Directly convert cyanide group to carboxyl group, to prepare carboxylic acid, which is widely used in medicine, environmental protection, chemical industry, food additive, textile, agricultural production and other fields.
There are 40 kinds of Nitrilase(NIT) products (Number as ES-NIT-101~ES-NIT-140) developed by SyncoZymes. ES-NIT is a useful tool to catalyze the synthesis of chiral carboxylic acids through regio- and stereoselective hydrolysis of a wide range of aliphatic and aromatic nitriles.

Catalytic reaction type:

Nitrilase NIT2

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Product Detail

Product Tags

Product Information:

Nitrilase NIT
Enzymes Product Code Specification
Enzyme Powder ES-NIT-101~ ES-NIT-140 a set of 40 Nitrilase, 50 mg each 40 items * 50mg / item, or other quantity
Screening Kit (SynKit) ES-NIT-4000 a set of 40 Nitrilase, 1 mg each 40 items * 1mg / item


★ High substrate specificity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

Instructions for use:

➢ Normally, the reaction system should include substrate, buffer solution (optimum reaction pH) and ES-NIT.
➢ All the ES-NITs can be tested respectively in the reaction system above or with the NIT Screening Kit (SynKit NIT).
➢ All kinds of ES-NITs corresponding to various optimum reaction conditions should be studied individually.
➢ High concentration Substrate or product with may inhibit ES-NIT’s activity. However, the inhibition can be relieved by batch addition of substrate.

Application Examples:

Example 1(Synthesis of nicotinic acid) (1):

Nitrilase NIT3

Example 2(Synthesis of (R)-(-)-mandelic acid) (2):

Nitrilase NIT4

Example 3(Synthesis of baclofen) (3):

Nitrilase NIT5

Example 4(Synthesis of glycolic acid) (4):

Nitrilase NIT6


Keep 2 years below -20℃.


Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.


1 Mathew C D, Nagasawa T, Kobayashi M, e tal. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Environ. Microbiol, 1991, 57(10): 3028.
3 Xu M Z, Ren J, Gong J S, e tal. Chin. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel A J, Petrillo K L, e tal. Biotechnol. Bioeng., 2008, 99(3): 717.

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