Nitrile reductase (NRED)
Short Description:
About Nitrile reductase
There is 3 kind of NRED enzyme product (Number as ES-NRED101~ ES-NRED103) developed by SyncoZymes. NRED can catalyze the reduction of nitrile group to amine. In the process of catalysis, NADPH is needed as hydrogen transporter.
Nitrile reductasecatalytic reaction type
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| Enzymes | Product Code | Specification |
| Specification | ES- NRED -101~ ES- NRED -103 | a set of 3 Nitrile Reductase, 50 mg each 3 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml Substrate
10 mg/ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
