★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.
➢ Normally, the reaction system should include substrate, buffer solution, enzyme, PLP, Mg2+.
➢ TAL should be added last into reaction system, after the pH and temperature been adjusted to the reaction condition.
Example 1(Synthesis of (2S, 3R)-p-methylsulfonyl phenylserine from p-methylsulfonyl benzaldehyde and threonine)(1):
Keep 2 years below -20℃.
Never contact with extreme conditions such as: high temperature, high/low pH and high concentration organic solvent.
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